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Background Research on non-target organ damage of biological pesticides has attracted much attention. Rotenone exposure may be far beyond the occupational environment, and the exposureduring pregnancy may be increased through bioaccumulation, fruit or vegetable residues, and other forms of oral intake. At present, the effects of rotenone on placental development and its mechanism are still unknown. Objective To investigate the developmental damage of rat placenta and evaluate the expression levels of glycogen synthase kinase (GSK-3β) and beta catenin (β-catenin) followed by rotenone exposure through the placental barrier during pregnancy, as well as to propose possible associated mechanisms. Methods Eighteen sexually mature SD female infertile rats without specific pathogens were selected and divided into three groups: blank control group (0.9% saline), corn oil group, and rotenone group (corn oil + 2 mg·kg−1 rotenone) by random number method, six female animals in each group. Another six male rats were selected and mated to the female rats at night with a female to male ratio of 3:1 per cage. Pregnant rats were given 0.9% saline, corn oil, and 2 mg·kg−1 rotenone preparation by isovolumetric gavage once daily for the entire gestation period (19 d), and their conditions were observed after the last dose. The pregnant rats were anesthetized, and the size of the placenta and blood perfusion were detected by ultrasound the next day of the last dose of rotenone. Then, 3 pregnant rats in each group were sacrificed immediately and the placenta and umbilical cord tissues were dissected. The remaining 9 pregnant rats gave birth naturally, and the fetuses were observed for developmental evaluation and weighed. The histopathological changes of umbilical cord and placenta were observed by hematoxylin-eosin staining. The reactive oxygen species levels of placenta tissues were detected by flow cytometry. The Ca2+-ATPase activity of placenta tissues was detected by colorimetric method. The localization and levels of GSK-3β and β-catenin expression of placenta were detected by immunohistochemistry. The p-GSK-3β/GSK-3β and p-β-catenin/β-catenin protein expression in placental tissues were measured by Western blotting. Results No injury or death was recorded during the pregnant rats receiving rotennon administration. Adverse pregnancy outcomes such as fetal absorption and postpartum stillbirth were found in the rotenone group, and the weight of the fetal mice decreased (P<0.05). The B-ultrasound showed disc-shaped placenta with a thick middle and thin edge, smooth fetal surface, rough maternal surface, visible placental lobules, granular echotexture of the placenta with comma-like echogenic densities, and chorionic plate showing deep indentations, no calcification, degeneration, or necrosis in each group. Compared with the corn oil group, the fetal surface diameter of the placenta was reduced in the rotenone group (P<0.05). The Doppler color ultrasound showed that interplacental blood flow was reduced in the rotenone group, while interplacental blood flow was abundant in the blank control and the corn oil groups. The hematoxylin-eosin staining results showed that smooth muscle cells in the umbilical cord tissues of rats were loosely arranged, with fuzzy nuclei and inflammatory infiltration in the rotenone group. The placental trophoblast cells were small in size, disorderly arranged with nuclear fragmentation and cytoplasm turbidity. The tissue reactive oxygen species level in the rotenone group was higher than those in the other two groups (P<0.05). The Ca2+-ATPase activity of placental tissues was reduced in the rotenone group (P<0.05). The immunofluorescence low-magnification observation showed that GSK-3β and β-catenin were expressed in placental tissue, weak fluorescence expression in the decidua basalis, strong fluorescence expression in the labyrinthine layer structure. The labyrinthine layer under high magnification showed that compared with the blank control group and the corn oil group, the brightness of β-catenin fluorescence expression in the rotenone group decreased (P<0.05), and the brightness of GSK-3β expression increased (P<0.05). The Western blotting results showed that the expression of β-catenin and p-GSK-3β proteins decreased (P<0.01), and the expression of GSK-3β protein increased (P<0.01) in the rotenone group. No significant expression of p-β-catenin protein was detected in the placenta tissue of each group. Conclusion Rotenone exposure during pregnancy induces placental hypoperfusion, growth retardation, and oxidative stress in rats, as well as down-regulation of β-catenin and p-GSK-3β protein expression, and up-regulation of GSK-3β protein expression, which may further lead to abnormal pregnancy and fetal restricted growth.
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Here, we took base construction of neurosurgery as example to discuss and analyze according to requirements and evaluation indexes of base construction in Xinqiao Hospital, and put forward the specific objectives, measures and implementations of base construction. Foremost, we summarized experiences and overcame shortcomings through interpreting and implementing scheme of our base construction, which would help to improve the construction of standardized residency training base in China.
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Objective:To evaluate the effect of total intravenous anesthesia combined with transversus abdominis plane block (TAPB) and rectus sheath block (RSB) in patients undergoing laparoscopic total hysterectomy.Methods:One hundred and twenty patients who required a laparoscopic total hysterectomy under total intravenous anesthesia from April 2019 to June 2020 in General Hospital of Eastern Theater Command were selected. The patients were divided into 4 groups by random digits table method with 30 cases each: total intravenous anesthesia group (C group), total intravenous anesthesia combined with TAPB group (T group), total intravenous anesthesia combined with RSB group (R group) and total intravenous anesthesia combined with TAPB and RSB group (S group). The narcotic (propfol and remifentanil) dosage, changes of resting visual analogue score (VAS) within 48 h after operation, pressure number of analgesia pump within 48 h after operation, anesthesia satisfaction score and incidence of emergence agitation were compared among 4 groups.Results:The remifentanil dosage, propfol dosage and pressure number of analgesia pump within 48 h after operation in S group were significantly lower than those in T group, R group and C group: (1.09 ± 0.23) mg vs. (1.49 ± 0.21), (1.47 ± 0.26) and (1.48 ± 0.23) mg, (543.53 ± 41.78) mg vs. (618.96 ± 37.21), (598.67 ± 37.86) and (607.87 ± 36.93) mg, (3.52 ± 2.03) times vs. (5.47 ± 1.83), (6.63 ± 2.08) and (9.77 ± 2.16) times, the anesthesia satisfaction score was significantly higher than that in R group and C group: (18.13 ± 3.22) scores vs. (21.43 ± 2.42) and (21.44 ± 2.56) scores, and there were statistical differences ( P<0.05). The resting VAS extubation and 12, 24, 48 h after operation in S group was significantly lower than that in C group and R group, the resting VAS 12, 24 and 48 h after operation was significantly lower than that in T group, and there were statistical differences ( P<0.05). The incidence of emergence agitation in S group was significantly lower than that in C group: 6.67% (2/30) vs. 26.67% (8/30), and there was statistical difference ( P<0.05). Conclusions:TAPB combined with RSB can reduce the narcotic dosage, reduce the incidence of emergence agitation and relieve perioperative pain in patients undergoing laparoscopic total hysterectomy. The effect is better than that of total intravenous anesthesia and total intravenous anesthesia combined with TAPB or RSB.
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Objective The choice of perioperative analgesic drugs for radical resection of colorectal cancer is controversial. The purpose of this paper is to investigate the efficacy of oxycodone hydrochloride injection on anesthesia and postoperative analgesia in patients with radical resection of colorectal cancer, to provide a basis for the selection of such surgical analgesics. Methods Between July 2017 and December 2017, selection of 38 patients with colorectal cancer underwent elective open surgical operation in the Eastern Theater General Hospital, the age range was 18～65 years, the BMI range was 18～25 kg/m2, the ASA was Ⅱ or Ⅲ grade, who were divided to two groups according to randomized, single blind and controlled methods, the oxycodone group was group O, the sufentanil was group S, each group had 19 cases. Oxycodone 0.3 mg/kg (group O) and sufentanil 0.3 μg/kg (group S) were given at general anesthesia induction in both groups.The anesthesia maintenance medication was consistent in both groups. When sewing skin, the group O were given oxycodone 0.15 mg/kg, the group S were given sufentanil 0.15 μg/kg , and who were given PCIA postoperative, the group O were given oxycodone 1 mg/kg and Azhasi 10 mg, while the group S were given sufentanil 2.5 μg/kg and Azhasi 10 mg, which were diluted with normal saline to 100 mL. The NRS score postoperative and the Ramsay sedation scores were recorded when extubating（T1）, 4 hours after operation（T2）, 12 hours after operation（T3）, 24 hours after operation（T4） and 48 hours after operation（T5）in two groups ; the plasma cortisol, MAP(mean arterial pressure) and HR(heart rate) before and after intubation in two groups were recorded; the extubation time, first bowel ventilation time, PCIA compressions and adverse reactions in two groups were recorded. Results The age, weight, anesthesia duration had no significant difference in two groups (P> 0.05). Compared with group S, the NRS scores were obvious decreased in group O at all time points（P 0.05). Compared with before intubation, the MAP were both decreased at intubation and after intubation 5min in group O (P 0.05) . Conclusion Compared with sufentanil, oxycodone was safer and more effective on general anesthesia induction and postoperative analgesia in radical resection of colorectal cancer.
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Objective To investigate the role of Shh-PARP-1 signaling pathway in the protective effects of tea polyphenols against the fatty acid-induced islet microvessel endothelial function injury. Methods Mouse islet microvessel endothelial MS-1 cells were used in this study, and the cells were divided into normal control group, solvent group, fatty acid group (0. 25 mmol/L palmitic acid + 0. 5 mmol/L oleic acid), tea polyphenols group (25 μmol/L), fatty acid + tea polyphenols group, PARP-1 inhibitor (8 μmol/L BYK204165) + fatty acid group, PARP-1 inhibitor + fatty acid + tea polyphenols group, Shh inhibitor (2. 5 μmol/L cyclopamine) + fatty acid group, Shh inhibitor + fatty acid + tea polyphenols group and inhibitors of Shh and PARP-1 + fatty acid +tea polyphenols group. The changes of cell viability, apoptosis, nitric oxide (NO) synthesis and oxidative stress related indicators were examined in each group. Results After fatty acid treatment, the survival rate ofMS-1 cells was decreased, and the level of apoptosis was significantly increased (P0. 05). Conclusion Fatty acid can directly trigger islet microvessel endothelial function injury, and tea polyphenols shows a protective effect against the toxicity of fatty acid, which can be enhanced by inhibiting Shh-PARP-1 signal pathway.
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Objective To investigate the protective effects of kaempferol against the fatty acid-induced islet microvessel endothelial function injury and the role of poly (ADP-ribose) polymerases-1 (PARP-1). Methods Mouse islet microvessel endothelial MS-1 cells were divided into normal control group, solvent (DMSO) group, fatty acid group (0. 25 mmol/L palmitic acid+0. 5 mmol/L oleic acid), kaempferol group (50 µmol/L), fatty acid + kaempferol group, PARP-1 inhibitor (8 µmol/L BYK204165)+fatty acid group and PARP-1 inhibitors+fatty acidVkaempferol group. The changes of cell viability, apoptosis, nitric oxide (NO), nitric oxide synthase (NOS) and oxidative stress related indicators were examined in each group. Results After treatment with fatty acid, the survival rate of MS-1 cells was significantly decreased and the apoptosis rate was significantly increased (P<0. 05); meanwhile, fatty acids also increased NO production and promoted the activities of the total NOS (tNOS), inducible NOS (iNOS) and constitutive NOS (cNOS) in the MS-1 cells (P<0. 05). Treatment with fatty acid also significantly increased the lipid peroxidation products-malondialdehyde (MDA), while significantly decreased the levels of antioxidants, glutathione (GSH) and superoxide dismutase (SOD) (P<0. 05); and t also increased the mRNA and protein expression of PARP-1, iNOS and cNOS (P <0. 05). Kaempferol significantly attenuated the toxic effects of fatty acidsconcerning all the detected indicators (P<0. 05). Moreover, pretreatment with PARP-1 inhibitor (BYK204165) for 1 h markedly enhanced the protective effects of kaempferol, and all the detected parameters were similar to those of the control group(P<0. 05). Conclusion Fatty acid can directly trigger islet microvessel endothelial function injury, and kaempferol shows a protective effect against the toxicity of fatty acid. Inhibition of PARP-1 can significantly promote the protective effects of kaempferol.
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OBJECTIVE:To establish a method for the simultaneous determination of paeoniflorin,baicalin and imperatorin in Urticaria pill. METHODS:HPLC was performed on the column of Alltima C18 with mobile phase of methanol-water-Phosphoric ac-id(55∶45∶0.2,V/V/V)at a flow rate of 1.0 ml/min,the detection wavelength was 230 nm for paeoniflorin,280 nm for baicalin and 300 nm for imperatorin,column temperature was 30 ℃,and the volume injection was 10 μl. RESULTS:The linear range was 5.40-54.0 μg/ml for paeoniflorin(r=0.999 8),11.29-112.9 μg/ml for baicalin(r=0.999 7)and 24.95-249.5 μg/ml for imperatorin (r=0.999 9),respectively;the linit of quantitation were 5.4、11.2、30.0 ng,the lirnit of detection were 1.8、2.8、7.5 ng;RSDs of pre-cision, stability and reproducibility tests were lower than 2%;recoveries were 95.88%-98.33%(RSD=0.95%,n=6), 96.86%-99.96%(RSD=1.20%,n=6)and 98.07%-100.55%(RSD=0.92%,n=6),respectively. CONCLUSIONS:The method is simple and accurate with strong specificity and good reproducibility,and can be used for the simultaneous determination of paeoni-florin,baicalin and imperatorin in Urticaria pill.
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Objective: To investigate the effect of novel epidermal growth factor receptor variant type A (EGFRvA) on migration of human glioma U87MC cells, and to explore its possible mechanism. Methods: The lentiviral expression vector pWPT-GFP [recombined with green fluorescent protein (GFP) gene, as the blank control], pWPT-EGFRwt (recombined with ECFR wild-type gene, as the negative control) and pWPT-EGFRvA (recombined with ECFRvA gene) was respectively co-transfected with packaging vector psPAX2 and pMD2.G into 293T cells to produce lentiviral particles. Subsequently, the lentiviral particles were separately infected into U87 MG cells to establish U87MG GFP, U87MG EGFRwt and U87MG EGFRvA cell lines. The overexpression levels of EGFRwt and EGFRvA in U87MG EGFRwt and U87MG EGFRvA cells were detected by flow cyctometry (FCM) and Western blotting. The migration capability of U87MG cells after transfection was investigated by Transwell migration test. The real-time fluorescent quantitative-PCR (RFQ-PCR) was performed to validate the varied mRNA expression levels of 52 genes which had been screened out in three types of cells by microarray. Then the protein expression levels of 4 tumor metastasis-related genes which had significantly varied mRNA expression levels in RFQ-PCR results were confirmed by Western blotting. Results: After U87 MG cells were stably infected with lentivirus particles, U87 MG EGFRwt and U87MG EGFRvA cells overexpressed EGFRwt and EGFRvA, respectively. The migration capability of U87MG EGFRvA cells was stronger than that of U87MG EGFRwt cells (P < 0.001). The expression levels of FBLN2 and ROBOI mRNAs were higher in U87MG EGFRvA cells than those in U87MG EGFRwt cells (both P < 0.05), whereas the expression levels of CDH13 and SOX2 mRNAs were the opposite (both P < 0.05). U87MG EGFRvA cells had a significantly lower expression levels of CDH1 3 and SOX2 proteins which were related to tumor metastasis (both P < 0.05). Conclusion: Overexpression of EGFRvA can promote the migration of U87MG cells through regulating the expressions of FBLN2, ROBO1, CDH13 and SOX2 genes in tumor cells.
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<p><b>OBJECTIVE</b>To investigate the presence of β-amyloid peptide (Aβ) deposition in the cerebellum and the expression of related miRNAs in the cerebellum of a mouse model of Alzheimer disease.</p><p><b>METHODS</b>Twelve 12-month-old APPswe/PSδE9 double transgenic mice and 12 wild-type C57 mice were sacrificed and the brain tissues were taken for examination. The right hemisphere was stained with Congo red to observe the deposition of amyloid substances, and from the left hemisphere, the hippocampus and the cerebellum were dissected for detecting the expression of miRNA-135a-5p, miRNA-298-5p, miRNA-466b-3p and miR-669f-3p using real-time PCR.</p><p><b>RESULTS</b>Congo red staining revealed the presence of Aβ deposition in both the hippocampus and the cerebellum of the transgenic mice but not in the control mice. Real-time PCR showed a significantly lower expression of the 4 miRNAs in the hippocampus in the transgenic mice than in the control mice (P<0.05). The expression of miRNA-135a-5p, miRNA-298-5p, and miR-669f-3p in the cerebellum was significantly lower in the transgenic mice than in the control mice (P<0.05). The expression of miRNA-298-5p and miR-669f-3p in the hippocampus was significantly lower than that in the cerebellum of the transgenic mice (P<0.05).</p><p><b>CONCLUSION</b>β deposition also occurs in the cerebellum of APPswe/PSδE9 double transgenic mice, and its formation might be related to the down-regulation of miRNA-135a-5p, miRNA-298-5p, and miR-669f-3p.</p>
Subject(s)
Animals , Mice , Alzheimer Disease , Metabolism , Amyloid beta-Peptides , Metabolism , Cerebellum , Metabolism , Disease Models, Animal , Hippocampus , Metabolism , Mice, Inbred C57BL , Mice, Transgenic , MicroRNAs , MetabolismABSTRACT
<p><b>BACKGROUND</b>In recent years, computers have been intensively used at home. In contrast to paper-based books and documents, computer screen is self-illuminated with larger screen-background luminance difference, which greatly induces visual discomfort. To compensate for that, one effective solution is to offer an additional background luminance. In this study, we investigated whether and to what extent additional background lighting is needed in looking at a computer display comfortably.</p><p><b>METHODS</b>In this study, 60 healthy children aged from 9 to 12 years and 58 adults aged from 21 to 39 years participated in the experiments. They were asked to choose their most preferred background luminance intensities at three screen luminance levels. The data were analyzed by Matlab (R2012b) and SPSS 20.0.</p><p><b>RESULTS</b>Both children and adult participants selected a non-dark background as their comfortable lighting condition when watching a computer display (children: t (59) = 22.0, P < 0.01, adults: t (57) = 15.5, P < 0.01). Comparatively, children preferred brighter background in general ( F (1,116) = 7.0, P < 0.01). More importantly, participants' preferred background luminance levels were linearly correlated with screen luminance intensities (children: slope = 0.97, R(2) = 0.98; adults: slope = 0.38, R(2) = 1.00).</p><p><b>CONCLUSION</b>These results indicate that varying background luminance to maintain screen-background luminance ratio is beneficial to human visual comfort.</p>
Subject(s)
Adult , Child , Female , Humans , Male , Young Adult , Computers , Contrast Sensitivity , Physiology , Lighting , MyopiaABSTRACT
<p><b>OBJECTIVE</b>To detect the expression of signal transducer and activator of transcription 3 (STAT3) and P-STAT3 in the brain of the APPswe/PS δE9 double transgenic mouse model of Alzhaimer's disease (AD) and investigate their possible role in AD.</p><p><b>METHODS</b>APPswe/PS δE9 double transgenic mice and control mice were examined for cerebral STAT3 and P-STAT3 expressions using immunothistochemistry.</p><p><b>RESULTS</b>STAT3 and P-STAT3 were expressed in the different regions of mouse brain. In the transgenic mice and the control mice, the positivity rates of STAT3 were 93.75% and 87.50% in the cerebral cortex, 87.50% and 43.75% in the basal forebrain, 81.25% and 37.50% in the hippocampus, and 62.50% and 0.00% in the cerebellum, respectively, showing significant differences between the mice in the STAT3 expressions in the basal forebrain, hippocampus and cerebellum (P<0.05). The positivity rates of P-STAT3 in the two groups were 0.00% and 0.00% in the cerebral cortex, 68.75% and 0.00% in the basal forebrain, 62.50% and 12.50% in the hippocampus, and 43.75% and 0.00% in the cerebellum, respectively, showing also significant differences in the basal forebrain, hippocampus and cerebellum (P<0.05). The expression of STAT3 was positively correlated with that of P-STAT3 in transgenic AD mice (P<0.05).</p><p><b>CONCLUSION</b>STAT3 and P-STAT3 are highly expressed in the basal forebrain, hippocampus and cerebellum in transgenic AD mice and may participate in the pathological process of AD.</p>
Subject(s)
Animals , Mice , Alzheimer Disease , Metabolism , Cerebellum , Metabolism , Cerebral Cortex , Metabolism , Disease Models, Animal , Hippocampus , Metabolism , Mice, Transgenic , STAT3 Transcription Factor , MetabolismABSTRACT
<p><b>OBJECTIVE</b>To detect the expression of miRNA-135a-5p, miRNA-135a-2-3p, miRNA-298-5p, miRNA-466b-3p and miR-669f-3p in the brain tissue of the APPswe/PS δE9 double transgenic mouse model of Alzheimer's disease using real-time PCR.</p><p><b>METHODS</b>Six-month-old APPswe/PS δE9 double transgenic mice and wild-type C57 mice of the same species were examined for the expressions of miRNA-135a-5p, miRNA-135a-2-3p, miRNA-298-5p, miRNA-466b-3p and miR-669f-3p in the brain tissue using real-time PCR.</p><p><b>RESULTS</b>The relative expression levels of the 5 miRNAs in the transgenic versus the wild-type mice were 0.73∓0.27 vs 1.08∓0.58, 2.47∓6.15 vs 1.65∓0.67, 0.72∓0.14 vs 1.31∓0.73, 0.57∓0.34 vs 1.06∓0.35, and 0.63∓0.26 vs 1.02∓0.18, respectively, showing significance differences in the expressions of miRNA-135a-5p, miRNA-298-5p, miRNA-466b-3p, and miR-669f-3p between the two groups (P<0.05).</p><p><b>CONCLUSIONS</b>miRNA-135a-5p, miRNA-298-5p, miRNA-466b-3p and miR-669f-3p are expressed differentially in APPswe/PS δE9 double transgenic mice, suggesting their important roles in the pathogenesis of Alzheimer disease.</p>
Subject(s)
Animals , Mice , Alzheimer Disease , Genetics , Metabolism , Brain , Metabolism , Disease Models, Animal , Mice, Inbred C57BL , Mice, Transgenic , MicroRNAs , Genetics , Metabolism , Real-Time Polymerase Chain Reaction , MethodsABSTRACT
<p><b>OBJECTIVE</b>To observe the changes of miRNA expression profiles in APPswe/PSδE9 transgenic mice and explore the possible roles of miRNA in the pathogenesis of Alzheimer's disease.</p><p><b>METHODS</b>Using miRNA chip technique, we examined the miRNA expression in the brain tissue of 6-month-old APPswe/PSδE9 transgenic mice, with age-matched wild-type mice as the control group.</p><p><b>RESULTS</b>Twelve miRNAs showed differential expressions by more than two folds in APPswe/PSδE9 transgenic mice, namely miRNA-135a, miRNA-135a-2*, miRNA-298, miRNA-466b-3p, miR-669-3p, miR-142-5p, miR-144, miR-466f-3p, miR-466g, miR-200a, miR-200b and miR-96. Five miRNAs were significantly down-regulated in the transgenic mice, including miRNA-135a, miRNA-135a-2*, miRNA-298, miRNA-466b-3p, and miR-669-3p.</p><p><b>CONCLUSION</b>The 5 down- regulated miRNA may play important roles in the pathogenesis of AD in APPswe/PSδE9 transgenic mice.</p>
Subject(s)
Animals , Mice , Alzheimer Disease , Genetics , Metabolism , Disease Models, Animal , Gene Expression Profiling , Mice, Transgenic , MicroRNAs , Genetics , Metabolism , Oligonucleotide Array Sequence Analysis , TranscriptomeABSTRACT
ObjectiveTo evaluate the effect of enteral ecoimmunonutrition (EIN) on hepatic injury in swine with acute necrotizing pancreatitis (ANP).MethodWith 20 pigs,swine model of ANP was induced by injecring 5％ sodium taurocholate into the pancreatic duct.24 hours after the ANP models were established,2 pig were dead,and the remaining 18 pigs were randomly divided into total parenteral nutrition (TPN) group ( n =6),elemental enteral nutrition (EEN) group ( n =6 ),and EIN group ( n =6 ),and received nutrition support for 8 days.The level of plasma endotoxin and serum concentrations of AST,ALT,TNF-α,and IL-6 were measured at different time points ( before model establishment,24 hours after model establishment,and the 1,2,4,8 days of nutrition support).All the pigs were sacrificed after the 8-day nutrition support,and ascites quantity,hepatic malondialdehyde (MDA),superoxide dismutase (SOD) were measured.The mRNA expression of TNF-α and IL-6 in hepatic tissue were detected by RT-PCR.Pathologic changes of the livers were analyzed.Results24 hours after the ANP models were established,the plasma endotoxin,AST,ALT,TNF-α,IL-6 in EIN group were significantly higher than the levels before model establishment ( all P ＜ 0.01 ),but not significantly different from the 9ther 2 groups.After 8 days of nutrition support,the above 5 indexes in EIN group were significantly lower than those in TPN group and EEN group ( all P ＜ 0.05 ).In addition,the ascites quantity,hepatic concentration of MDA,the expression of TNF-α and IL-6,and hepatic pathological score in EIN group were lower than those of TPN group and EEN group,while hepatic SOD activity was higher in EIN group than in the other 2 groups (all P ＜ 0.05 ).ConclusionEarly EIN could attenuate hepatic injury in ANP from various aspects.
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@#ObjectiveTo investigate the safety and clinical value of treadmill exercise testing for the patients with acute myocardial infarction in the early recovery. Methods36 patients with acute myocardial infarction performed treadmill exercise testing with Bruce protocol 15～57 d after infarction. ResultsThe outcome of 19 cases was positive, and the outcome of other 16 cases was negative. 1 case couldn't finish the testing, whose outcome could not be analyzed because his exercise time was too short. The average cardic fuction was (5.42±3.12) METs. ConclusionIt is safe that the patients with acute myocardial infarction performed treadmill exercise testing early.
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Objective To investigate the inhibitory mechanism of rabeprazole on acid secretion of parietal cells in rats. Methods Seventy two Spraque-Dawley rats were divided into control group(normal saline), low dose of rabeprazole group (rabeprazole 10 mg/kg) and high dose of rabeprazole group (rabeprazole 20 mg/kg) with 24 each. Four rats in each group were treated at 1 h, 2 h, 4 h, 6 h, 12 h and 24 h, respectively, pH value and activity of H+-K+-ATPase were tested by NaOH titrimetric method and chromatometry, respectively. The changes of ultrastructure of parietal cells were observed by electron microscope. Results Compared with control group(1.97±0.30), pH value in low (3.37± 0.97)and high (5.96±0.26)dose groups were significantly increased(P<0.01), and the activity of H+-K+-ATPase was inhibited obviously at 1 h after administration(3.28±0. 41 vs 1.47±0.27 and 0.92±0.07, P<0.05). Those differences could be found even at 12 h(P<0.01). The ultrastructural changes of parietal cells were consistent with the increase of pH value and decreased activity of H+-K+-ATPase. The significant difference was found in acid depression and excellent time between low and high dose of rabeprazole groups. Conclnsions The uhrastructural changes of parietal cells and the activity of H+-K+-ATPase can accurately reflect the acid secretion in rats. Rabeprazole has powerful and rapid effects in inhibiting acid secretion which correlates with the dosage.
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Objective To evaluate the effect of ecoimmunonutrition on systemic inflammatory response in swine with acute necrotizing pancreatitis (ANP). Methods Swine model of ANP was induced by retrograde injection of 5% sodium taurocholate and trypsin into the pancreatic duct. 24h after the ANP models were established, eighteen swine were randomly divided into total parenteral nutrition (PN) group (n = 6), enteral nutrition (EN) group (n = 6), and ecoimmunonutrition (E1N) group (n = 6). Each of the three nutrition formulas was given to each group respectively for eight days. The level of plasma endotoxin and serum concentrations of TNF-α, IL-1β, IL-6 and IL-10 were measured before, 24 h after the induction of ANP and 1,2, 4, 8 d after nutrition support. Results 24 hours after the ANP models were established, the serum level of plasma endotoxin, TNF-α, IL-1β, IL-6 and IL-10, NF-κB activities in EIN group were ( 1.85 ± 0.18) EU/L,(461.59 ± 128.25 ) pg/ml, ( 185.49 ± 58.81 ) pg/ml, ( 354.26 ± 34.63 ) pg/ml, ( 110.32 s 25. 18 ) pg/ml,(51.06 ± 2.27 )%, respectively, which all were significantly higher than those before the ANP models were established, but there were no difference among three groups. Eight days after nutrition support, those levels in EIN group were (1.48 ±0.16 )EU/L, (30.11 ±9.12)pg/ml, (20.17 ±7.04)pg/ml, (36.42 ±7.24) pg/ml and ( 89.46 ± 13.54 ) pg/ml, (9.06 ± 0. 17 ) %, respectively, which all were significantly lower than those of TPN and EEN group ( P ＜ 0. 05 ). The ration of IL-10/IL-6 was 2.51 ± 0.42 in EIN group, and 2.28 ± 0. 19 before the ANP models were established. Conclusions EIN could attenuate endotoxemia, decrease NF-κB activities and serum concentrations of cytokines and maintained the balance of pro- and anti-inflammation.
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Objective To evaluate the effect of ecoimmunonutrition on maintenance of the mucosal barrier function in pigs with severe acute pancreatitis(SAP).Methods Twenty pigs were injected with5% sodium taurocholate and trypsin into the pancreatic duct to establish SAP models.Twenty four hours later,18 suvival pigs were randomly given parenteral nutrition(PN group,n=6),elemental enteral nutrition(EEN group,n=6),or ecoimmunonutrition(EIN group,n=6)for 8 days.The serum amylase,endotoxin and intestinal permeability were determined on different times.All pigs were sacrificed 8days later,and their venous blood,mesenteric lymph nodes(MLN),liver,lung,spleen and pancreas specimens were cultured quantitatively and qualitatively with standard techniques.The morphology of small bowel was observed and pathological changes of the pancreas was analyzed.Results Serum amylase of all groups were obviously elevated,but there was no significant difference among them(P＞0.05).The plasma endotoxin and intestinal permeability were lower in EIN group[(1.50±0.18)EU/L and(42.8±20.0)×10-3 respeetively]compared to EEN group[(1.98 4±0.20)EU/L and(67.4±23.0)×10-3 respectively]and PN group[(3.96±0.40)EU/L and(197.2±47.4)×10-3.respectively](all P value＜0.05).The incidence and the magnitude of bacterial translocation in EIN group were lower than those in PN and EEN groups.However,the ileum mucosa thickness,villus height,crypt depth and the rate of the normal intestinal villi in EIN group were(398.27±52.93)μm,(269.72±41.66)μm,(681.98±58.33)μm and 79%,respectively,but in PN group were(218.32±35.81)μm,(145.76±23.34)μm,(376.20±48.23)μm and13%,respectively and in EEN group were(305.70±42.72)μm,(192.52±38.17)μm,(507.31±68.23)μm and 47%,respectively.Conclusions EIN can maintain the integrity of intestinal mucosal barrier and decreasing intestinal bacterial and endotoxin translocation in SAP pigs.
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OBJECTIVE To investigate the clinical characteristics and prevention of urinary tract infections in chronic renal failure(CRF) patients.METHODS An analysis of pathogens and clinical characteristics of 96 hospitalized patients with CRF complicating urinary tract infections was performed.RESULTS Among CRF patients complicating urinary system infections,the most common pathogens were Escherichia coli(51.03%),Klebsiella pneumoniae(12.50%),Staphylococcus epidermidis(11.46),etc.There were no significant difference between symptom group and non-symptom group.But they were obviously more among senile patients over 60 years old in the asymptomatic group,accounted for 67.50% and significantly higher than that in symptomatic group(39.29%,P
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Objective:To investigate the clinical effect of Niaodukang-enema liquid on chronic renal failure. Methods: 112 cases were divided into treatment group and control group randomly. The patients of treatment group were given oral Medicinal Charcoal Tablets and Niaodukang-enema liquid(120ml) for enema at high position,56 cases. The patients of contral group were given oral Medicinal Charcoal Tablets only, 56cases. The renal function was observed before and after treatment in both groups.Results :The distinct effective rates and total effective rates in treatment group were obviously better than that in contral group (P